Expression and Purification of enzymatically active forms of the human lysyl oxidase -
نویسندگان
چکیده
Genome Research Center for Birth Defects and Genetic Diseases, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, 388-1, Pungnap-2 Dong, Songpa-Gu, Seoul 138-736, Korea Cardiovascular Research Center, John A. Burns School of Medicine, University of Hawaii, 1993 East West Road, Honolulu, HI 96822, USA School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea
منابع مشابه
Expression and purification of enzymatically active forms of the human lysyl oxidase-like protein 4.
The lysyl oxidase-like protein 4 (LOXL4) is the latest member of the emerging family of lysyl oxidases, several of which were shown to function as copper-dependent amine oxidases catalyzing lysine-derived cross-links in extracellular matrix proteins. LOXL4 contains four scavenger receptor cysteine-rich domains in addition to the characteristic domains of the LOX family, including the copper-bin...
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Lysyl oxidase activity was assayed in urea extracts of a number of human tissues, proving to be highest in skin. Antibodies to human placental lysyl oxidase completely inhibited the activity of crude lysyl oxidase from all the human tissues studied, with no significant differences in the amounts of antiserum required for 50% inhibition. By contrast, marked differences were found in this value b...
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Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Transferase (GST)) were engineered on the N-terminus of mature lysyl oxidase. Total enzyme yields were determined to be 1.5 mg for the Nus-A tagged e...
متن کاملExpression of lysyl oxidase (LOX) in human placental amnion in preterm labor
Purpose: The proper function of amnion is necessary for a normal pregnancy and delivery to occur. Understanding the change of amnion between term and preterm labor leads to a better method of diagnosis and prevention for preterm labor. Lysyl oxidase (LOX) catalyzes a series of reactions about the formation of cross-linking of collagen fibrils. Human amniotic tissue, containing a large amount of...
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Accumulating evidence indicates that aldosterone plays a critical role in the mediation of oxidative stress and vascular damage. NADPH oxidase has been recognized as a major source of oxidative stress in vasculature. However, the relation between NADPH oxidase in aldosterone-mediated oxidative stress in endothelial cells remains to be ascertained. The present study aimed to investigate the rel...
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